| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199503 |
| Chromosome: | chromosome 2 |
| Location: | 5122930 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105050 | (1 of 1) PTHR34810:SF1 - DNA-BINDING PROTEIN BIN4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAGGCCGGCGGCGGCGGAAGCTCGAACA |
| Internal bar code: | GGCGCAGGAACGTGAAGCGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 467 |
| LEAP-Seq percent confirming: | 99.8519 |
| LEAP-Seq n confirming: | 2022 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCGTAGCTCATGTCCTC |
| Suggested primer 2: | GTTGACACAAACGCAACCAC |