| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.199522 |
| Chromosome: | chromosome 6 |
| Location: | 4832220 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278350 | AGD1 | (1 of 1) 1.3.1.78 - Arogenate dehydrogenase (NADP(+)) / TyrAAT2; Arogenate/prephenate dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCGGCGGTGGGGTACGGCTGCACGGTA |
| Internal bar code: | CGCGGTTCTACACAGAACGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1022 |
| LEAP-Seq percent confirming: | 97.7476 |
| LEAP-Seq n confirming: | 4600 |
| LEAP-Seq n nonconfirming: | 106 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAATCAAGGGCCGTAACCA |
| Suggested primer 2: | TGAAGTTGAAGTACGGCGTG |