Insertion junction: LMJ.RY0402.199526_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.199526
Chromosome:chromosome 6
Location:7367614
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g299150 PHC9,PHC26 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 26 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTCTGGATCACTTGGGCCTGAGCTTTGT
Internal bar code:TGCACTCCAGTATCGAACTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:311
LEAP-Seq percent confirming:99.6433
LEAP-Seq n confirming:2514
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGACCACAGAGCCAAACA
Suggested primer 2:CAGGTTTTGCCGGGTACTTA