| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199554 |
| Chromosome: | chromosome 10 |
| Location: | 6520350 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466750 | SAT1 | (1 of 1) PTHR23416//PTHR23416:SF2 - SIALIC ACID SYNTHASE-RELATED // SERINE ACETYLTRANSFERASE 5; Serine O-acetyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGTGACAAGTATCCACCTGGTAGTTGG |
| Internal bar code: | CGGCCAACGGCACCGTTCTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 821 |
| LEAP-Seq percent confirming: | 95.7833 |
| LEAP-Seq n confirming: | 2635 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTCGCCCTACTTATTGT |
| Suggested primer 2: | CTGCGTGTGATTAGGCAAGA |