| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.199571 |
| Chromosome: | chromosome 3 |
| Location: | 8351901 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g198800 | (1 of 1) PTHR24078:SF169 - DNAJ HOMOLOG SUBFAMILY C MEMBER 17 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCGAGGAGGAGCACAGGGCGTTCTTG |
| Internal bar code: | AAGTGTAAGTTAGATGGATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1022 |
| LEAP-Seq percent confirming: | 99.78 |
| LEAP-Seq n confirming: | 6351 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGACAGGCCCAGAAGTAGC |
| Suggested primer 2: | ACCTACAGAATCCCGTGTCG |