Insertion junction: LMJ.RY0402.199577_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.199577
Chromosome:chromosome 1
Location:2440109
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g013650 (1 of 3) PF15612 - WSTF, HB1, Itc1p, MBD9 motif 1 (WHIM1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCGCCTCCCAGTAACCCTCCGCGTCCCG
Internal bar code:CTAGGCCCGTCTCGGACTCACC

Confirmation - LEAP-Seq

LEAP-Seq distance:1070
LEAP-Seq percent confirming:97.5273
LEAP-Seq n confirming:1341
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTAAGGAGGAGACACAGGCG
Suggested primer 2:CGCACCAACTACTCAAAGCA