Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.199596 |
Chromosome: | chromosome 13 |
Location: | 2008838 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g576740 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTAGCGTCCTATGGCATTGACCGCACGC |
Internal bar code: | CCAGGGGCCGCCCCGCGGCTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 654 |
LEAP-Seq percent confirming: | 96.9404 |
LEAP-Seq n confirming: | 1204 |
LEAP-Seq n nonconfirming: | 38 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTGTGGGTGAGTCCACTTT |
Suggested primer 2: | CCAGCTGAACAATAGCGACA |