| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199610 |
| Chromosome: | chromosome 17 |
| Location: | 1638183 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708050 | (1 of 1) PTHR23257:SF297 - PROTO-ONCOGENE SERINE/THREONINE-PROTEIN KINASE MOS | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCAGCGCCTTTTCGAAAACATGACACAC |
| Internal bar code: | CCGTGCGTAGGATCACAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 80.3717 |
| LEAP-Seq n confirming: | 692 |
| LEAP-Seq n nonconfirming: | 169 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACCACCAAGTCTTCAGG |
| Suggested primer 2: | GGAGACGGAACAGAGTACGG |