Insertion junction: LMJ.RY0402.199611_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.199611
Chromosome:chromosome 6
Location:7724163
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g301951 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTGAACGTTTCTCGTGAATATTCCTCGC
Internal bar code:TACTGCGTGTATCGTGCACTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:614
LEAP-Seq percent confirming:99.7252
LEAP-Seq n confirming:3266
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GACTTGAACACCTGCAGCAA
Suggested primer 2:TTCCTTGACGCCTAACTGCT