Insertion junction: LMJ.RY0402.199635_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.199635
Chromosome:chromosome 11
Location:1835888
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467750 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTAGAAACGTGCCTTGCGGCGAGCCAGGG
Internal bar code:CACGTCCCGTAGATTGTCTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:805
LEAP-Seq percent confirming:99.3151
LEAP-Seq n confirming:145
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCTCAGGCTATTGCGTTGTG
Suggested primer 2:GCTATCGAGCCCTGTTGAAG