Insertion junction: LMJ.RY0402.199640_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.199640
Chromosome:chromosome 10
Location:1287395
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g427300 PF24,RSP2 Radial Spoke Protein 2; (1 of 1) PTHR23356//PTHR23356:SF5 - DPY30-RELATED // SUBFAMILY NOT NAMED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACCGAACATCGAACTTGTCGCCCAGACC
Internal bar code:GGGGGGGAGGGTCCACCGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:560
LEAP-Seq percent confirming:99.5546
LEAP-Seq n confirming:894
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AAACTTGACTTGGTGGGTGC
Suggested primer 2:CGCTAGAGGACAGGAAGTGG