Insertion junction: LMJ.RY0402.199664_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.199664
Chromosome:chromosome 13
Location:4542179
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g603225 (1 of 2) K03924 - MoxR-like ATPase (moxR) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGGGTGCATGCCGCGGCGGGGCGGCTGG
Internal bar code:TATCTAGTTACCACACTGCCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:499
LEAP-Seq percent confirming:99.1304
LEAP-Seq n confirming:114
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACCACAAAAACACACAC
Suggested primer 2:GATTGCGATGGGAATGAACT