| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199687 |
| Chromosome: | chromosome 9 |
| Location: | 2757758 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389200 | UMM8 | UbiE/COQ5 methyltransferase family protein; (1 of 1) 2.1.1.288 - Aklanonic acid methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATCCTACAGTACTTTACGGATGTCACAT |
| Internal bar code: | GGACTTGACCAGGTGAACCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 274 |
| LEAP-Seq percent confirming: | 99.7364 |
| LEAP-Seq n confirming: | 1135 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAATTTTGCAGGTTGGCGT |
| Suggested primer 2: | GAGAGTATGGAGGTGCTGGC |