| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.199702 |
| Chromosome: | chromosome 14 |
| Location: | 1110674 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615100 | MSR1,MSRB1B | (1 of 3) K07305 - peptide-methionine (R)-S-oxide reductase (msrB); Methionine sulphoxide reductase 1B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGGATTGGTTCAACGTTAACCCCCACC |
| Internal bar code: | GTGGGTGCTCCCGGTGTCCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 642 |
| LEAP-Seq percent confirming: | 99.6091 |
| LEAP-Seq n confirming: | 6371 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACGCACTTCCTGTGGTCC |
| Suggested primer 2: | CCAGGTCCTAGTCCTGGTCA |