| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.199749 |
| Chromosome: | chromosome 3 |
| Location: | 4623774 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177200 | CRB3 | RNA-binding protein C3, CELF family, subunit of CHLAMY1; (1 of 2) K13207 - CUG-BP- and ETR3-like factor (CUGBP, BRUNOL, CELF) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACGGCAGGGCAGTTGCTGGGGTTGGTGG |
| Internal bar code: | TTAATTAGATACGTGATAGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1018 |
| LEAP-Seq percent confirming: | 99.3261 |
| LEAP-Seq n confirming: | 2653 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTGGGGTTGACTCAGAT |
| Suggested primer 2: | AGCGAAGGGCTAGGTAGGAG |