| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199766 |
| Chromosome: | chromosome 9 |
| Location: | 5850747 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402256 | (1 of 3) 3.4.11.1 - Leucyl aminopeptidase / Peptidase S | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTGCGGGTTCTGAGGGCAGGCGGGGAG |
| Internal bar code: | CGTCTCTTAGGGGTCGGCAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 919 |
| LEAP-Seq percent confirming: | 99.773 |
| LEAP-Seq n confirming: | 3956 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTGCCCTGTTATCTTTC |
| Suggested primer 2: | AAGCTGAGACGAGAGCAAGC |