| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.199777 |
| Chromosome: | chromosome_12 |
| Location: | 5288881 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g528850 | DLX1,DLC3 | Flagellar outer dynein arm 16 kDa light chain, LC3 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTACGAGGGGCGAAGGGAATGTGCTTGGAC |
| Internal bar code: | AGATCTTGCGCGTGTGTTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 555 |
| LEAP-Seq percent confirming: | 95.5254 |
| LEAP-Seq n confirming: | 1409 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGCACAGGGCATCACTA |
| Suggested primer 2: | ACCAGCAAGTGCTGTCATTG |