| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.199802 |
| Chromosome: | chromosome 12 |
| Location: | 3040181 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500650 | RNB2 | (1 of 1) PTHR23355//PTHR23355:SF39 - RIBONUCLEASE // SUBFAMILY NOT NAMED; chloroplast RNase II | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGGGCGCGCACTAGCGTGGGGGCCGAG |
| Internal bar code: | GCTTTTTCGGTCCCTGACTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 93.9286 |
| LEAP-Seq n confirming: | 263 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGCTTTGGTGTACGATT |
| Suggested primer 2: | AACCACCTGCTCTCCAGCTA |