| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.199817 |
| Chromosome: | chromosome 12 |
| Location: | 9201813 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541850 | (1 of 1) 3.6.1.7 - Acylphosphatase / Acylphosphate phosphohydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTTGCCAACATCGAGGCCGGCCACAACT |
| Internal bar code: | CACAGACCGATTCTAGTTTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 465 |
| LEAP-Seq percent confirming: | 99.0519 |
| LEAP-Seq n confirming: | 2194 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGGTGGGGGATAGAGAT |
| Suggested primer 2: | CCCATTATGGTTATGGACGC |