| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199823 |
| Chromosome: | chromosome_7 |
| Location: | 5019582 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g347050 | RSP7 | Radial spoke protein 7 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGGTGGGGGAGGGCCGAGGAGGTTTGGGA |
| Internal bar code: | TTGGGTACGGGGGCGCATGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 713 |
| LEAP-Seq percent confirming: | 88.3624 |
| LEAP-Seq n confirming: | 2741 |
| LEAP-Seq n nonconfirming: | 361 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAACTCGACCTACCGCACC |
| Suggested primer 2: | GAAAGCTGGTCTTCCGACTG |