| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199873 |
| Chromosome: | chromosome 1 |
| Location: | 2484858 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g014100 | (1 of 1) PTHR14614//PTHR14614:SF29 - HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN // PROTEIN FAM86A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGCCTAAATGGGTGCGACAGAAGAACGT |
| Internal bar code: | CACGAGGTCTAGTAGTACGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 322 |
| LEAP-Seq percent confirming: | 99.8491 |
| LEAP-Seq n confirming: | 1985 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCCTTGATTGTCCCTCA |
| Suggested primer 2: | CCGACGAGGAGTTGTTTGAT |