Insertion junction: LMJ.RY0402.199875_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.199875
Chromosome:chromosome 9
Location:6626790
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g408626 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCGAGTAGAGTGCGGGAAGGGGAGTCGG
Internal bar code:AAATCGAGGTGAAGCTTACATC

Confirmation - LEAP-Seq

LEAP-Seq distance:426
LEAP-Seq percent confirming:87.0588
LEAP-Seq n confirming:74
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CATCTTATGGCGCAGCTACA
Suggested primer 2:GTGCCTGGTTTGAGTGGTTT