| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.199920 |
| Chromosome: | chromosome_5 |
| Location: | 2953299 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g238280 | FAP205 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CACTTGCTCAGTCGCCAGCAGCCGCCAGGC |
| Internal bar code: | CGGACACAATAGACCGGAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 249 |
| LEAP-Seq percent confirming: | 93.4138 |
| LEAP-Seq n confirming: | 851 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATATACGGCTTTTCGGGA |
| Suggested primer 2: | GACGAGAAGGGGCAGTACAT |