| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.199937 |
| Chromosome: | chromosome 14 |
| Location: | 1942846 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621100 | (1 of 1) IPR000104//IPR001810//IPR025886 - Antifreeze protein, type I // F-box domain // Phloem protein 2-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTCTGATATGCGTGGGAGCAGTTGCAG |
| Internal bar code: | AAAGGAGGATCGTCTTTACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 99.512 |
| LEAP-Seq n confirming: | 2039 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGCCTATGGTATTTCGTT |
| Suggested primer 2: | TGTGGTCTGCTTCAGGTCAG |