| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.199941 |
| Chromosome: | chromosome 7 |
| Location: | 174160 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313350 | (1 of 3) PTHR22642:SF2 - AMIDOHYDROLASE FAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGATGCATAGCAGCAGCATCCCAGGTTCC |
| Internal bar code: | GTTTCGTGCGCGCCAGAAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 230 |
| LEAP-Seq percent confirming: | 34.2201 |
| LEAP-Seq n confirming: | 849 |
| LEAP-Seq n nonconfirming: | 1632 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCAAGAGCAATAGCGGT |
| Suggested primer 2: | TGGTATTGTGTGACTGGGGA |