Insertion junction: LMJ.RY0402.199950_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.199950
Chromosome:chromosome_14
Location:3762939
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
None

Insertion site details

Flanking sequence (orientation from cassette outwards):TTGATATCTTCGAAGGCCTACCCAAGTCGC
Internal bar code:GGTGTCAAGGGTGTCCAGACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:393
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCTTTTGCACGTCAGTGT
Suggested primer 2:AGGGACAGTTTTCCAACACG