Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.199950 |
Chromosome: | chromosome 17 |
Location: | 3315959 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g723300 | (1 of 18) K08824 - cyclin-dependent kinase-like [EC:2.7.11.22] (CDKL) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCTTGGCTGGGCGGGAGGGAGCGACGG |
Internal bar code: | CGGGTCCTTCCAGGCCAATTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 651 |
LEAP-Seq percent confirming: | 99.6468 |
LEAP-Seq n confirming: | 5642 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAATGGACTACGCGACCAAT |
Suggested primer 2: | CCTGTGATGGAACACGTGAC |