Insertion junction: LMJ.RY0402.199979_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.199979
Chromosome:chromosome 10
Location:5289187
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g457400 (1 of 28) PTHR31600//PTHR31600:SF2 - FAMILY NOT NAMED // TINY MACROCYSTS PROTEIN B-RELATED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCTGCCCCTCCAGCTCGGATGCTTTGTA
Internal bar code:CAGACTGTCTCGAGGGGCTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:654
LEAP-Seq percent confirming:99.7186
LEAP-Seq n confirming:3898
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTTCCACCACCAGGTCTT
Suggested primer 2:CATTATGGAACGATTTGGGG