Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.199983 |
Chromosome: | chromosome 13 |
Location: | 3313047 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g586450 | NAT31 | N-acetyltransferase; (1 of 5) PF13508 - Acetyltransferase (GNAT) domain (Acetyltransf_7) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCATTCTGAGGCCTGTTGAGGAGAGAG |
Internal bar code: | CATGATTGGCTCTAATACTTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 939 |
LEAP-Seq percent confirming: | 99.3154 |
LEAP-Seq n confirming: | 2031 |
LEAP-Seq n nonconfirming: | 14 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAACCATAGAGTTGGCACGG |
Suggested primer 2: | GTGCCCGATGTAAGACCTGT |