Insertion junction: LMJ.RY0402.200000_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.200000
Chromosome:chromosome 17
Location:1452004
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g706700 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACATGTAGTCGTGACGTCCTGTGATCGAA
Internal bar code:AGGTCCTACGGAGGAGAACAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:961
LEAP-Seq percent confirming:99.616
LEAP-Seq n confirming:1816
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ACGAGATGAGGGGTGTTACG
Suggested primer 2:GTCCGTATGTTGCTCCCACT