| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.200003 |
| Chromosome: | chromosome 7 |
| Location: | 1692618 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325650 | (1 of 3) PF10601 - LITAF-like zinc ribbon domain (zf-LITAF-like) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGCATGAACCCACACGTTCACCAGGCCC |
| Internal bar code: | CCCTGGCAGGGCTATCGATCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.5775 |
| LEAP-Seq n confirming: | 1414 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGATTTCATGGAGGAGGG |
| Suggested primer 2: | ACATGGACTTGCCATCATCA |