| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.200018 |
| Chromosome: | chromosome 7 |
| Location: | 4130151 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342750 | (1 of 1) K18106 - D-galacturonate reductase (GAAA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCATTTCGTGTGGCTTGCAAAACACCGC |
| Internal bar code: | TGACACGAACAATGTATGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 99.4995 |
| LEAP-Seq n confirming: | 994 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGCAGTTGCCTTTCCTC |
| Suggested primer 2: | CGTGTGCATGTGTGTGTGTT |