| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.200019 |
| Chromosome: | chromosome 2 |
| Location: | 6237237 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g114100 | PDE22 | 3\',5\'-cyclic-nucleotide phosphodiesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACACGCATACGCATGGCATGGTCACAT |
| Internal bar code: | AAACATCTTTCCTACCCCGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 652 |
| LEAP-Seq percent confirming: | 99.7713 |
| LEAP-Seq n confirming: | 2617 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAGCACCTGATCCGAACG |
| Suggested primer 2: | AGCCCGCAACTCTAAACTCA |