| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.200021 |
| Chromosome: | chromosome 1 |
| Location: | 6812770 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049117 | 5'UTR | ||
| Cre01.g049132 | (1 of 1) PTHR23097//PTHR23097:SF105 - TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER // SUBFAMILY NOT NAMED | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCTCTTGCTGTCGGGACCCGTTGTATT |
| Internal bar code: | AGGTAGCAGGAGGGGGTCGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 658 |
| LEAP-Seq percent confirming: | 99.7847 |
| LEAP-Seq n confirming: | 927 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCGACCTCCACAACAAAG |
| Suggested primer 2: | CATAAGCTTCAAAGCCCTCG |