| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.200041 |
| Chromosome: | chromosome 7 |
| Location: | 1665393 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325350 | KIN4-3,KIN10A,KIN10-1 | Kinesin motor protein; (1 of 4) K10395 - kinesin family member 4/21/27 (KIF4_21_27) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTAATAATTTGGTTCGCGCTCTCCTCT |
| Internal bar code: | CCGTTATTCATGTTTTATCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 98.6584 |
| LEAP-Seq n confirming: | 956 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCGGCTTGACCGTAGGTA |
| Suggested primer 2: | TCGGTAGTGTCCATTAGGGC |