| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.200058 |
| Chromosome: | chromosome 9 |
| Location: | 4154182 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393954 | FAP106 | Flagellar Associated Protein 106; (1 of 1) PTHR21490:SF0 - ENKURIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATTGGGGGGGGCGGTCACTTTGGGCTCA |
| Internal bar code: | TTGAGAAGAGTGGTGTGTATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1011 |
| LEAP-Seq percent confirming: | 99.8095 |
| LEAP-Seq n confirming: | 3668 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGAGTGTGGACAGTGCT |
| Suggested primer 2: | CTATTGGTGTGCGTGTGGTC |