| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.200090 |
| Chromosome: | chromosome 14 |
| Location: | 4009773 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633350 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTACACACACACACACACACACACGTAC |
| Internal bar code: | GTCCGATCACCTCCGAGGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 289 |
| LEAP-Seq percent confirming: | 49.9138 |
| LEAP-Seq n confirming: | 2027 |
| LEAP-Seq n nonconfirming: | 2034 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGTCAGAACCAGGTCAT |
| Suggested primer 2: | CAGTAGCAGCAGTAGCAGCG |