| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200091 |
| Chromosome: | chromosome 12 |
| Location: | 3104837 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500050 | (1 of 19) PF00397 - WW domain (WW) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAAGCAACCCGACCAACCCCACGCGCA |
| Internal bar code: | CGTTGGGCTAACATCCACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1103 |
| LEAP-Seq percent confirming: | 99.8049 |
| LEAP-Seq n confirming: | 3070 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAGCATCCATTTCTGGCT |
| Suggested primer 2: | AAGGAACGGACAGAAGAGCA |