Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.200092 |
Chromosome: | chromosome_3 |
Location: | 4757791 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g178100 | Lipoxygenase | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGGGCAGCACCAGGCGTGAAGCAGCATCAC |
Internal bar code: | GGCCCCCGGCCAGGGCCCATAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1138 |
LEAP-Seq percent confirming: | 99.7335 |
LEAP-Seq n confirming: | 5988 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGAACGTGTCAACCTGTCCC |
Suggested primer 2: | ACAAGCTGTACGTGTTCCCC |