| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200095 |
| Chromosome: | chromosome 1 |
| Location: | 3295741 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020850 | CYG49 | (1 of 4) PTHR11920//PTHR11920:SF248 - ADENYLATE AND GUANYLATE CYCLASES // SUBFAMILY NOT NAMED; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGGTGCCGGGCGGACGTCTGGCTGCTG |
| Internal bar code: | ACATTCAAGCTGCTGAGGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1047 |
| LEAP-Seq percent confirming: | 99.6511 |
| LEAP-Seq n confirming: | 4284 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGTCTCATCACTAGCCTC |
| Suggested primer 2: | GTCAACCCATAGTGACGGCT |