| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200100 |
| Chromosome: | chromosome 2 |
| Location: | 6256537 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g114250 | MEN2 | Naphthoate synthase; (1 of 1) 4.1.3.36 - 1,4-dihydroxy-2-naphthoyl-CoA synthase / o-succinylbenzoyl-CoA 1,4-dihydroxy-2-naphthoate-lyase (cyclizing) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCCACACCACAGGGCCGCCAGGCCTACA |
| Internal bar code: | CAAGCAGAGGGCACGCAGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 79 |
| LEAP-Seq percent confirming: | 65.3333 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCAACTGGAGACGGAGAC |
| Suggested primer 2: | CGTACCGCTCTTGTCTCCTC |