Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.200101 |
Chromosome: | chromosome 16 |
Location: | 2950513 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g664150 | PDE18 | (1 of 3) 3.1.4.17//3.1.4.53 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase // 3',5'-cyclic-AMP phosphodiesterase / cAMP-specific phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACAACGCGCCTGGCACCACATCTGGAA |
Internal bar code: | GAGCTGAGGTGGACTTGGCGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 841 |
LEAP-Seq percent confirming: | 98.5752 |
LEAP-Seq n confirming: | 3321 |
LEAP-Seq n nonconfirming: | 48 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAAAGCCACACACACACACC |
Suggested primer 2: | TACACCGTGAACCCTGTCAA |