| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.200127 |
| Chromosome: | chromosome 15 |
| Location: | 278818 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640901 | (1 of 1) IPR000104//IPR029033 - Antifreeze protein, type I // Histidine phosphatase superfamily | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACACACTGCACCGCACCCGCCGCTGCACA |
| Internal bar code: | CGGGGTATGGCCCGCGCGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 329 |
| LEAP-Seq percent confirming: | 99.3865 |
| LEAP-Seq n confirming: | 324 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAATCGTGTTTAGGGCGAC |
| Suggested primer 2: | GCCCTGAAGGTACAATCCAA |