| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.200132 |
| Chromosome: | chromosome_16 |
| Location: | 6910640 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g676645 | FAP200 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGAGACGGATGCCACGGGCTACGTGGGCCT |
| Internal bar code: | CGCTTCATTTGACATGAAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 131 |
| LEAP-Seq percent confirming: | 99.5407 |
| LEAP-Seq n confirming: | 1517 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTCCCCTACCCTGTACG |
| Suggested primer 2: | TTCAAAGGTCTACGCTGGCT |