| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.200139 |
| Chromosome: | chromosome 16 |
| Location: | 6885678 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676421 | TOC1,PRR1 | Pseudo Response Regulator 1; (1 of 1) PTHR26402//PTHR26402:SF465//PTHR31319 - RESPONSE REGULATOR OF TWO-COMPONENT SYSTEM // TWO-COMPONENT RESPONSE REGULATOR-LIKE APRR1-RELATED // FAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACAGGCAGGTGCAGCCCAGGCAGCCCT |
| Internal bar code: | GGGTACGCCTTCCCTTGAGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 85.8247 |
| LEAP-Seq n confirming: | 1332 |
| LEAP-Seq n nonconfirming: | 220 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCGCGATCTTATTGGTGT |
| Suggested primer 2: | CGGGAAGTGAAACATGGACT |