| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.200175 |
| Chromosome: | chromosome 9 |
| Location: | 4710024 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397031 | (1 of 6) 5.3.99.5 - Thromboxane-A synthase / Thromboxane synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGCGCGTTGACGTAACCTTGCTTTGTG |
| Internal bar code: | CGAAATGGGAAGACGCGCAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 303 |
| LEAP-Seq percent confirming: | 99.8561 |
| LEAP-Seq n confirming: | 694 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCATACAAGTGTGTGGG |
| Suggested primer 2: | AAACATCACCTGGGTAAGCG |