| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.200185 |
| Chromosome: | chromosome 6 |
| Location: | 1221813 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257700 | RAD18 | Putative E3 ubiquitin ligase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGAGCTGATGGCGGCAGCAGCCGCGGT |
| Internal bar code: | ACGCCCCCGGGAGATACTAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 50.4638 |
| LEAP-Seq n confirming: | 272 |
| LEAP-Seq n nonconfirming: | 267 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGGTGGGTTAGTTGGT |
| Suggested primer 2: | CACTCGTCTTGCTTGAGCTG |