| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.200185 |
| Chromosome: | chromosome 10 |
| Location: | 5769171 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461250 | NSF1 | (1 of 1) K06027 - vesicle-fusing ATPase (NSF) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTAATCACACAGAATGCCAAGTCGTGA |
| Internal bar code: | AGTGGTATGTTAGCTGCGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 177 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGAACTTATCCGAGCCTG |
| Suggested primer 2: | CCTTGGCATACACCATACCC |