| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.200194 |
| Chromosome: | chromosome 12 |
| Location: | 7298928 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557500 | (1 of 1) IPR000104//IPR002073//IPR003607//IPR006189//IPR023088 - Antifreeze protein, type I // 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain // HD/PDEase domain // CHASE domain // 3'5'-cyclic nucleotide phosphodiesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTTGGTTATGCGCTGGGTGGCTGCTGG |
| Internal bar code: | CGCTGGGCCGGCGACGGCTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 201 |
| LEAP-Seq percent confirming: | 99.6147 |
| LEAP-Seq n confirming: | 5947 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTAGTCACGCCACTGAA |
| Suggested primer 2: | GGCATGCCGTTACGATACTT |