| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.200203 |
| Chromosome: | chromosome 8 |
| Location: | 4858608 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384700 | DMA3 | (1 of 1) PF06325 - Ribosomal protein L11 methyltransferase (PrmA) (PrmA); Putative DNA methylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTTTGGGCAGGTTGGTGGACATGTGCT |
| Internal bar code: | CCTGGACTTATAAGGCGCCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 57.1099 |
| LEAP-Seq n confirming: | 743 |
| LEAP-Seq n nonconfirming: | 558 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGGAGCTCTTGTGTA |
| Suggested primer 2: | GACAGAGAGGCACACGTTCA |